Assembling an actin cytoskeleton for cell attachment and movement

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Assembling an actin cytoskeleton for cell attachment and movement.

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Actin cytoskeleton: Setting the pace of cell movement

Eukaryotic cells have many proteins that cap the barbed ends of actin filaments. Manipulation of their cellular concentration leads to changes in cell motility rates, actin dynamics and signal transduction reactions.

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Progestogens regulate endothelial actin cytoskeleton and cell movement via the actin-binding protein moesin.

The endothelial effects of progestogens are poorly investigated. Actin remodeling and cell movement are fundamental for endothelial function and are controlled by the actin-binding protein moesin. In this work, we studied the effects of progesterone and medroxyprogesterone acetate (MPA) on actin remodeling, moesin activation and cell movement in human endothelial cells. Our findings show that p...

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Arginylation of beta-actin regulates actin cytoskeleton and cell motility.

Posttranslational arginylation is critical for mouse embryogenesis, cardiovascular development, and angiogenesis, but its molecular effects and the identity of proteins arginylated in vivo are unknown. We found that beta-actin was arginylated in vivo to regulate actin filament properties, beta-actin localization, and lamella formation in motile cells. Arginylation of beta-actin apparently repre...

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Movement of yeast cortical actin cytoskeleton visualized in vivo.

Fusion proteins between the green fluorescent protein (GFP) and the cytoskeleton proteins Act1p (actin), Sac6p (yeast fimbrin homolog), and Abp1p in budding yeast (Saccharomyces cerevisiae) localize to the cortical actin patches. The actin fusions could not function as the sole actin source in yeast, but fusions between the actin-binding proteins Abp1p and Sac6p complement fully the phenotypes ...

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ژورنال

عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

سال: 1998

ISSN: 0167-4889

DOI: 10.1016/s0167-4889(98)00080-9